Social anxiety disorder is one of the most common and debilitating mental health disorders, leading to a reduced quality of life and increased comorbid psychiatric disorders. However, despite decades of research linking social anxiety disorder to negative interpretation bias, the tendency to interpret ambiguous social cues negatively, results on this relationship are inconclusive. This suggest that moderating factors might be involved. This study investigated the involvement of perceived social support as a possible moderator. 118 first year university students completed questionnaires measuring fear of negative evaluation as an indication of social anxiety, and perceived social support from friends. They then completed an ambiguous facial rating task measuring negative interpretation bias. Hayes’ PROCESS Macro was used to calculate correlation and moderation effects. Results showed that fear of negative evaluation was not significantly related to negative interpretation bias, and perceived social support did not moderate this relationship. Surprisingly, conditional effects suggested an increased effect of fear of negative evaluation on negative interpretation bias at higher levels of perceived social support. Although these effects did not reach statistical significance, this would imply the opposite of the proposed hypothesis. Future research should focus on replicating these results with higher sample sizes above clinical thresholds of social anxiety disorder. These results might further increase the understanding of perceived social support as a moderator for the relationship between social anxiety disorder and negative interpretation bias and improve future treatment strategies.